The present invention relates to a quorum biosensor. More particularly, the present invention relates to a biosensor for use in detecting short chain N-acyl homoserine lactone synthase inhibitor, characterized in that, the biosensor is a N-acyl homoserine lactone-producing Escherichia coli comprising an arabinose-dependent expression plasmid, its method of manufacturing and method of use thereof. The present invention provides a novel approach for development of short chain N-acyl homoserine lactone synthase inhibitor biosensor that is capable of detecting specific compounds, in particular non-antibiotic compounds, which can be used in controlling quorum sensing-mediated bacterial infection.