Skip to main content

Method Of Cloning, Expression, Purification Of Serine Protease(S) From Lignosus Rhinocerus

Potential Commercialised fa-solid fa-cart-arrow-down violet
Reg. ID : 17022
Comments

Huraian

Disclosed are a method for cloning genes of a plurality of serine proteases (SPs). The method includes a step of extracting ribonucleic acid (RNA) from Lignosus rhinocerus (TM02). The plurality of serine protease (SPs) is obtained from Lignosus rhinocerus (TM02). The plurality of serine protease (SPs) include a GME4347 RNA and a GME8711 RNA. The method includes a step of performing a reverse transcription-polymerse chain reaction (RT-PCR) to synthesize a complementary DNA (cDNA) to GME4347 RNA and the GME8711 RNA. The method includes a step of cloning the complementary DNA (cDNA) to GME4347 RNA and the GME8711 RNA into a pGEM-T Easy Vector System I and transformed into Escherichia coli BL21 (DE3) cells.

Contact Person/Inventor

Name Email Contact Phone
Um Centre Of Innovation And Enterprise (Umcie) umcie@um.edu.my 013-2250151 / 03-79677351

Intellectual Property

Type of IP Registration ID
1 Copyright CRLY00000159/CRLY00000118

Award

Award Title Award Achievement Award Year Received
0 0 0

Komen

LOG IN or REGISTER to post comments.

Star Rating

Star Rating
Masih tiada undi

Contact Form