The objective of this paper is to discuss the application Iris Recognition Algorithm for scanning the presence of the cholesterol in human body with analysis human iris. This research manoeuvre the iris recognition method done by John Daugman's and Libor masek's with extends it in medical purpose for detecting diseases (cholesterol) according to iridology. This analysis use MATLAB program to execute Iris Recognition Algorithm for localize the iris segment (sclera and limbic). The shape rectangular is selected after transform the circular shape of iris. MATLAB programming gives the result from this analysis whether cholesterol is presence or not.