A method for detecting presence of Cytomegalovirus in a biological sample comprises the steps of isolating DNA sample from the biological sample; mixing an effective amount of the isolated DNA sample into a reaction reagent containing fluorescent moieties attached oligonucleotides probe SEQ. No 1 and SEQ. No 2 to form a mixture; subjecting the mixture for thermo cycling of 30 to 45 cycles including denaturation at 90 to 95°C for 25 to 35 second followed by annealing at 58 to 62°C for 35 to 45 second then extension at 66 to 74°C for 30 to 90 second to produce amplicons derived from Cytomegalovirus; verifying presence of Cytomegalovirus in the biological sample via detection of the produced amplicons in the mixture using melting curve analysis.