The present invention relates to a polymer coated nylon membrane for use in biosensing application. More particularly, the present invention relates to a nylon membrane for protein immobilization in a dengue virus detection characterized in that the nylon membrane comprising a polymer of methyl methacrylate and methacrylic acid coated thereon and method of manufacturing thereof. The present invention provides a novel approach for fabrication of a cost-effective and flexible class of bioreceptor platforms which can be used for enhanced dengue virus detection. The methyl methacrylate and methacrylic acid combination coating helps to create a unique structure that offers considerably high surface area for biomolecular immobilization.