Skip to main content

A method of detecting and identifying dengue virus serotype

Potential Commercialised fa-solid fa-cart-arrow-down violet
Reg. ID : 16773
Comments

Description

A method of detecting presence of a dengue virus in a biological sample comprises the steps of isolating ribonucleic acid specimen from the biological sample; subjecting the isolated ribonucleic acid specimen to a reverse transcription-loop-mediated isothermal amplification in a single-tube reaction using primers with nucleic acid sequence as set forth in SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, and SEQ ID NO. 8 throughout a constant temperature involving a forward outer primer, a backward outer primer, a forward inner primer, and a backward inner primer; and ascertaining presence of the dengue virus in the biological sample upon acquiring an amplicon of predetermined sizes. The disclosed method further comprises a primer with nucleic acid sequence as set forth in SEQ ID NO. 9 as the loop primer. The disclosed primers are derived from the 3 untranslated region of the dengue virus genome.

Contact Person/Inventor

Name Email Contact Phone
Um Centre Of Innovation And Enterprise (Umcie) umcie@um.edu.my 013-2250151 / 03-79677351

Intellectual Property

Type of IP Registration ID
1 Patent Filed PI2020003838
2 Trademark TM2021009753

Award

Award Title Award Achievement Award Year Received
0 0 0

Comment

LOG IN or REGISTER to post comments.

Star Rating

Star Rating
No votes yet

Contact Form