Our invention focuses on the utilization of loop-mediated isothermal amplification (LAMP) technology for the detection of Leptospira by targeting two important genes of Leptospira, one for the detection of all Leptospira and another for the detection of only the pathogenic strains of Leptospira. This will allow not just the detection but also differentiation of both pathogenic and saprophytic Leptospira simultaneously, further improving both its specificity and sensitivity.