The invention of this research is the production of cellulase enzymes with higher activity having potentials in the production of biothanol through hydrolysis of the cellulosic materials such as rice straw, empty fruit bunches (EFB). Currently, cellulase enzymes has potential industrial application in the textile industry for finishing of the cellulosic materials, detergent industry to provide cleaning and fabric-care benefits such as the brightening of colour in faded garments and paper industry in the pulp management and de-inking. Palm oil mill effluent (POME) has been used as a major medium due to the availability and least expensive through bioconversion by filamentous fungi to produce the enzymes. A total of 25 filamentous fungi were screened using POME in which 16 strains were isolated and purified from oil palm industrial residues and 9 strains were of laboratory stock.