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Real-Time Viral Detection Method and Kit For Infectious Hypodermal and Haematopoietic Necrosis Virus

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Reg. ID : 16770
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Huraian

A method for quantitatively detecting infectious hypodermal and haematopoietic necrosis virus (IHHNV) in a biological sample, comprising the steps of obtaining a primer pair having nucleotide sequences set forth in SEQ ID NO: 1 and SEQ ID NO: 2, and a fluorescent probe having a nucleotide sequence set forth in SEQ ID NO: 3; amplifying a non-structural protein coding region of the virus in the biological sample by a real-time polymerase chain reaction (PCR) using the primer pair and the fluorescent probe; and determining the presence and quantity of the virus in the biological sample with reference to a positive control plasmid.

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Um Centre Of Innovation And Enterprise (Umcie) umcie@um.edu.my 013-2250151 / 03-79677351

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