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A method for synthesizing a recombinant antigen from a truncated antigenic hypothetical lipoprotein of Burkholderia Pseudomallei

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Reg. ID : 16763
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Huraian

The present invention relates to a method for synthesizing a recombinant antigen for use in detecting or preventing melioidosis, comprising the steps of designing truncation of a polynucleotide sequence having a nucleotide sequence as set forth in SEQ ID NO: 1, which encodes an antigenic hypothetical lipoprotein derived from Burkholderiapseudomallei, based on antigenic site prediction; amplifying the truncated polynucleotide sequence designed to obtain a truncated polynucleotide fragment with antigenic sites;cloning the truncated polynucleotide fragment to obtain a recombinant plasmid; and transforming the recombinant plasmid in a bacterial host to express the truncated antigenic hypothetical lipoprotein fragment, which is to be used as the recombinant antigen.

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Um Centre Of Innovation And Enterprise (Umcie) umcie@um.edu.my 013-2250151 / 03-79677351

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