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Bacteriocin From Enterococcus Faecium C1

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Reg. ID : 16748
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Huraian

The present invention relates to the bacteriocin and method to purify bacteriocin molecules (BacC1) isolated from Enterococcus faecium C1. Bacteriocin BacC1, comprising acid amino sequence of GPXGPXP (G = glycine, P = proline, X = hydroxyproline), produced by the bacteria strain is useful for inhibiting growth of selected food-spoilage microorganisms and/or food-borne pathogens, hence increasing the shelf life of food products. The bacteria strain of the present invention, Enterococcus faecium C1, demonstrates properties of non- pathogenic, non-haemolytic, non-antibiotic resistant, and may be used as a probiotic bacteria in food products. The method to purify bacteriocin involves precipitating the bacteriocin using ammonium sulfate; separating the precipitate using fraction hydrophobic column; and centrifuging the precipitate. A chromatography separation involving reverse- phase high-performance liquid chromatography (HPLC) is used to further purify the bacteriocin.

Contact Person/Inventor

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Um Centre Of Innovation And Enterprise (Umcie) umcie@um.edu.my 013-2250151 / 03-79677351

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