THE PRESENT INVENTION PROVIDES A METHOD OF ISOLATING, CULTURING, PROLIFERATING AND MAINTENANCE OF THE MESENCHYMAL STEM CELLS (MSCs) DERIVED FROM HUMAN PHERIPHERAL BLOOD. THE PERIPHERAL BLOOD IS A (CLINICALLY ACCESSIBLE) POTENTIAL SOURCE OF MSCs. THE METHOD USED IN THE PRESENT INVENTION IS RELIABLE, INVOLVED MINIMAL INVASIVE PROCEDURE, LESS TIME AND COST CONSUMING. MSCs IS ABLE TO ISOLATE, PURIFY, GREATLY MULTIPLY AND DIFFERENTIATE INTO CARTILAGE CELLS FOR THE USE OF TREATING CARTILAGE DEFECT. THUS, THE PRESENT INVENTION ALSO PROVIDES A POTENTIAL CELL THERAPHY TO REPAIR CARTILAGE DEFECTS FROM THE HUMAN BLOOD DERIVED CHODROGENIS-MSCs. FIGURE 2