White spot syndrome virus (WSSV) causes millions of dollars losses to the shrimp industry in Asia. The Fast Target white spot syndrome virus detection kit is the first kit to use single tube, single loading, nonstop approach to detect white spot syndrome virus (WSSV). The single tube method is completely different from the conventional nested PCR that involves two cumbersome tubes and multiple-step procedures. This novel single tube method for shrimp virus is the first in the world. This kit gives user a non-stop nested amplification -low risk of contamination, economical, fast and user friendly. The advantages of this kit is its sensitivity to detect as low as 10 copies of viral DNA target; able to indicate infection levels (light, moderate and heavy); and it has built-in negative and positive controls for reliable results.